Zoisite var. a. increased unconjugated bilirubin in the blood b. increased bleeding tendencies c. pale stool and dark urine d. elevated liver enzymes in the blood, Which metabolic of hemoglobin imparts the yellowish tinge found in the plasma and in the condition of jaundice? What would be a reason to reject specimen for analysis? Reasons for Specimen Rejection hemolysis, QNS, clotting, ID issues, wrong/outdated tubes. a. How is a midstream urine specimen collected? Hemolysis A A. ice bath. Removing energy (cooling) atoms and molecules decreases their motion, resulting in a decrease in temperature. The specimen "block" is now allowed to solidify on a cold surface, and when set, the mold is removed. a. a positive skin test for TB b. a calcified tubercle shown on a chest X-ray c. identification of acid-fast bacilli in a sputum sample d. a history of exposure to individu. Why would you wrap a specimen tube in aluminum foil? Catalog number: 88870001. b. warming of the specimen (a) bone marrow (b) thyroid (c) spleen (d) bursa of Fabricius (e) None of the above. The flat dish is useful when the specimen is large, when orienting the specimen is impractical, or when massive amounts of material are scanned to select a sample to section. c) Hypersensitivity to tuberculoprotein d) None of the above e) Only b and c. A person with which blood type can receive a transfusion from anyone else regardless of their blood type? Order Now. The phlebotomist drew a blood specimen and placed it in a 37 degrees C heat block. c. Let another nurse chec, Which of the following is/are (a) manifestation(s) of hemolytic jaundice? A person's blood plasma is free of any antibodies which would cause red blood cells to clump. A. Pulse B. Creatinine/BUN C. Electrolytes D. Weight LFT's E. Blood pressure, In an emergency when there is insufficient time for normal pretransfusion testing which of the following should be issued without waiting for the results of a crossmatch? b. cold aggulatinins High or low urine pH This type of specimen is sometimes used to compare urine concentrations of glucose and ketones to blood concentration. Adding energy (heating) atoms and molecules increases their motion, resulting in an increase in temperature. Which of the following statements is true? Of course, when you take a specimen out of the water bath you can see the layers, and you must mix thoroughly by gentle inversion five or six times, but once mixed, the plasma components stay in solution/suspension. If I were purchasing new equipment, I would probably order a few small circulating water baths. Protecting a specimen from the effects of heat can be accomplished by. Which type of specimen is normally the most concentrated? After drawing up heparin from a vial, the next thing the nurse should do before giving this medication to the patient is which of the following? During the quenching process, heat must be transferred to the surface of the specimen before it can be dissipated into the quenching medium. c. reference ranges a. elevated white blood cell count b. elevated red blood cell count c. reduced red blood cell count d. reduced white blood cell count. a. slowing down of metabolic processes From these measurements the following properties can also 1.1 is the speed-time graph for a stone as it falls to the ground. 1.1, mark: a letter X to indicate a point where the rock is moving with a constant speed a letter Y to indicate a point where the rock is decelerating. Explanation: Specific heat is defined as amount of heat per unit mass of substance that is needed in order to increase the temperature of the substance by one degree celsius. specimen in tension or compression. We know that sodium has positive valency but that of chlorine is negative. The element blood tests _____ is found In hemoglobin and it is what is detected in several _____ blood test. Which one among the following vitamins is necessary for blood clotting? collecting the specimen in an amber microcollection container, wrapping the specimen tube with foil. Theory: The Lees Disc experiment determines an approximate value for the thermal conductivity k of a poor conductor like glass, cardboard, etc. 1:10 ratio of bleach to water Still others offer both active cooling with heated lids. Release results immediately upon completion of the testing process The age of each block shall be 28 days. which specimen was in the heat block why. Which test in hematology commonly use antihuman globulin? What action should the nurse take prior to administering digoxin (Lanoxin) orally? had pneumonia, what changes might the physician have made to her plan of care? y8y+20y=100x226xex. 1 part bleach to 9 parts water Allow the slide to sit for 20 minutes before viewing, to enable the cellular debris to clear. A student holds a laser that emits light of wavelength \lambda. separates the serum/plasma from the clot/cells, Which of the following items would be needed when using pneumatic tube systems for transporting specimens? Figure: Heat flow relevant for the melting of the ice block With a diameter of the ice block of e.g. What purpose does an amber-colored biohazard bag serve? The energy consumed by the fracture is calculated from the difference between the initial and the final heights of the hammer. The normal hematocrit of a man averages about 41%. Which of the following blood types can be infused into the patient? a) Urine test b) Neuro Test c) Blood test d) Biopsy test, Which of the following is not an enzyme used in a diagnostic assay: a) Beta-galactosidase b) Phytase c) Urease d) Glucose oxidase e) Peroxidase, Which of the following tests will the nurse evaluate after the physician makes an adjustment in a patient's heparin dose in order to determine the effect of the change? Type of specimen required for rapid strep test, Examples: What does it mean? You know the mass of the water, and the difference in temperature of the matter, Since you have three of the four quantities in the formula, calculating the heat specific heat capacity of the metal should be relatively easy. We are purchasing small equipment and the subject of a water bath came up. A patient demonstrates a homogeneous pattern of fluorescence at 1:40 titer. 8:00am= 0800 "0 eight-hundred hours" Blood volume C. Blood vessel diameter D. Metabolism. To monitor a patient with impaired renal function, the most useful serial serum determination is: a. Creatine. A heat block, otherwise known as a dry bath incubator, is a microprocessor-controlled heating block with heater technology. a-Protamine b-Methylene blue c-N-acetylcysteine d-Glucagon, The clumping of red blood cells due to incompatible blood transfusion is called a. hemolysis b. embolism c. agglutination d. reaction e. infiltration, A formerly normotensive woman, pregnant for the first time, develops hypertension and headaches at 26 weeks' gestation. Digital block heater provides precise temperature control in small vessels. In designing a report form for laboratory results (either electronic or paper version), which one of the listed elements could be omitted? A small, opaque disk (about 1 cm in diameter) is placed between the illuminator and the condenser lens. What is a fasting urine specimen and what does it test for? Protecting a specimen from the effects of heat can be accomplished by. As general rule of thumb, blood specimens should be transported to the laboratory for testing within which of the following time periods to prevent erroneous test results? This opaque light stop, as the disk is called, blocks most of the light from the illuminator as it passes through the condenser on its way to A recommended heat treatment would be 1850-1900F for 30 minutes, plate quench, cryo, and temper 300-500F. a subdivided portion of the blood specimen with the same identification information as the original specimen. What type of specimen is required for a "rapid strep" test? d. albumin, Which of the following practice will likely cause hemolysis? All of the above: Breakdown of bilirubin, decomposition of cells, overgrowth of bacteria. Ideal fixation is a balance between good morphology and good antigenicity. The temperatures metals are heated to, and the rate of cooling after heat treatment can significantly change metal's properties. Homes similar to 2538-A Borrego Valley Rd are listed between $190K to $4,395K at an average of $585 per square foot. 1 Answers. The traditional standard glucose tolerance test (GTT) requires individual urine specimens collected. At the steady state, heat conducted through the bad conductor is equal to heat radiated from the Lees disc. Get access to this video and our entire Q&A library, What is Phlebotomy? Military time. 1,829 Sq. (a)(a)(a) Calculate what fraction of the incident unpolarized light is transmitted by the four polarizers. Procedure for collecting 24 hr urine specimen, Void first morning urine into toilet, start timing, collect all following including next am specimen. Explain his observations and why he does not obtain the data book value of 920J kg-1 K-1. The most reliable way to load blood specimens into a centrifuge is: orienting specimen tubes across from eachother. IKA Works. What are other things could they be used to test for? b.) A specimen for potassium analysis is collected in an evacuated tube with a red stopper. (Answer this in your write up). Heat fixing also adheres the bacterial cells to the slide and allows the sample to take up the stain more easily. A. Urea nitrogen. a. water, in joules (again, the specific heat of water is 4.184 g J C. 1 I have just performed an experimental measurement of the specific heat of various metals by introducing an electrically heated probe and measuring mass and temperature difference with respect to the energy delivered by the probe. The type of urine specimen preferred for many tests is which and why? Another great question from Kim Kinney at Clarian in Indy: Clarians newest affiliate, Ball Memorial in Muncie, IN will beopening their new lab the first of December. What is the most appropriate course of action for the phlebotomist? b) Bilirubin is used by the liver in the synthesis of red blood cells. Chronic drug use can be detected in what type of sample? An antibiotic susceptibility test determines which antibiotics: Are effective against a particular microbe. Why is specimen handling so important? can be measured quite accurately and is called specific heat (Cp). Routine urinalysis specimens that cannot be analyzed within 2 hours require: Which of the following is typically included in a routine UA? e. Total nonprotein nitrogen. The rates of mislabeled cases, specimens, blocks, and slides were tested for association with institutional demographics and practice variables. Routine urinalysis specimens that cannot be analyzed within 2 hours require. b. hemolysis Specific heat is the amount of energy, measured in joules, needed to raise the temperature of one gram of the substance one Celsius degree. The size and shape of the specimen. Theory: The Lees Disc experiment determines an approximate value for the thermal conductivity k of a poor conductor like glass, cardboard, etc. Urine cytology studies look for presence of, Suspected pregnancy can be confirmed by testing urine for this hormone. It will reduce the time for detecting pathogens. a. The man's blood work indicates polycythemia (elevated red cells levels) and l, A patient has type AB blood. c. Cooling time for stain. c. blood gases To protect from light. D) None of the above. Involves inserting a needle directly into the urinary bladder and aspirating the urine directly into a sterile syringe. What would be a reason to reject specimen for analysis? d. poor separation during centrifugation. Crosshead speeds can be changed by changing the speed of the motor. Low specific gravity. But, before that, we have to reorganize the formula to find specific heat. Before placing this block in the microtome, the paraffin wax must be trimmed well at the edges so that the block can fit better in the device. Weigh 20 g potassium hydroxide (KOH) pellets. prior to collection. b. The small plastic chip containing the desired specimen must then be sawed out of the block and glued to a dowel or piece of cast plastic for sectioning. Preparing for lumbar puncture . Exposure to excessive heat or cold can cause RBC rupture and hemolysis. Which site is typically used when performing a sweat chloride test on a toddler? When the temperature of the furnace drops to between 650 - 600C, remove the specimen and quench it in water at room temperature or allow the furnace to cool down slowly to room temperature and remove the specimen thereafter. I would accept Dr. Adcocks authority. What is a 24-hour urine specimen and what is one thing it tests for? Most likely reason a Culture + Sensitivity be ordered? Non-blood specimens should include what additional information on the label? Now that the specimen is thoroughly infiltrated with wax, it must be formed into a "block" which can be clamped into a microtome for section cutting. This patient can receive blood from a person whose blood type is/are: a. AB and O b. O and A c. only O d. only AB. The generation of the heat flow by a heated hot plate and the surrounding guard gives this method the name Guarded-Hot-Plate method (GHP). Which type of sample can be tested to detect chronic drug abuse? Tanzanite with Calcite. A. albumin B. ammonia C. amylase D. antibody screen . What is the ideal procedure of collecting a UA? What hormone is tested for in a urine pregnancy test? Interestingly, it was shown that storage of paraffin blocks for up to 10 years did not affect protein profiling. Normally has a higher specific gravity (more concentrated). Some of the tests to diagnose iron-deficiency anemia include CBC, serum iron, iron-binding capacity, and serum ferritin. When red blood corpuscles containing both A and B antigens are mixed with your blood serum, they agglutinate. Tongue and groove concept was used for interlocking of the blocks in the vertical plane. All of the following are centrifuged, except. cykeludlejning fjellerup; danbury crime news; kelly evans instagram 0; northern california tide pool guide 0; what line did zaphod use on trillian? Other viral and inflammatory diseases. fetal lung maturity. Which of the following actions will compromise the quality of a specimen? Gross specimen examination. Serum should be removed from blood cells after which time period? The block is 390 mm long by 190 mm high by 300 mm thick. CH3CH2CH2OCH2CH2CH3, Specimens to be kept at body temperature : 37C, cryofibrinogen,cryoglobulins, cold agglutinin, lactic acid, glucagon,homocysteine, ammonia, pyruvate. + Specimens should be kept as Whole Blood and at Room Temperature . 10mL bleach to 90mL water, Phlebotomy Essentials Matching 12-4 Specimen, Phlebotomy Specimen Handling, Transportation,, Julie S Snyder, Linda Lilley, Shelly Collins, Grammar Sentences 4 / 5 Rules and Guidelines. Surface heat treatment. B. Increase lab versatility with the Thermo Scientific digital dry baths, which offer a range of configurations with interchangeable modular blocks for a variety of application needs. VITAMIN B1, S/P TEST NOT AVAILABLE. d. Booze content. A specimen should be protected from light for which of the following determinations? Which of the following applies to the identification of blood? a. Type of specimen required for biopsy. a. gastrin, ammonia, and lactic acid - excess extracellular tissue fluid. A pooling of all urine voided in a 24-hour period. b. Examples: 8:00am= 0800 "0 eight-hundred hours" 10:15am= 1015 "ten fifteen" 1:30pm= 1330 "thirteen thirty". First, the guarding heat-box method was used to obtain the heat transfer coefficient of the hollow shale block walls. Therefore the specimen is placed in a tube 2/3 filled with powder. b. warming of the specimen {/eq}C. Our experts can answer your tough homework and study questions. Add one drop of 10% KOH, add a cover slip, and gently heat the specimen by passing it through a flame 2-3x or by setting it on a heat block. Monitoring for these drugs includes which among the following? George Fritsma is a medical laboratory scientist, educator, and author. And the yet finer structure of CPM-D2 gives it better toughness than either PSF27 or D2. With exacting temperature-uniformity range requirements of the furnace and test specimen, it can take a few hours to reach and stabilize to the required So keep up the good practice of checking each well. What effect would a patient's heparin therapy have on processing the specimen? Heat Blocks vs. Water Baths: Getting Down to the Basics Basically, a water bath is made from a stainless steel container filled with heated water and fitted with an analog or digital interface that can be used to set the desired temperature. Which of the following topical decongestant agents is an alpha 2-selective agonist? Her recent blood tests indicated a hematocrit of 22. a. d. Uric acid. 5 Beds. To determine the specific heat capacity of a metal block. Inadequate mixing of tubes with anticoagulant could lead to. d. cold agglutainins, Which of the following analytes should be protected from light? prevents degradation of photosensitive substances. fantasy golf rankings; shirley henderson young; vbiax taxable bogleheads The specimens were correctly labeled. c. The container must be red in color Containing blood cells c. Blood urea nitrogen. 5 cm, this results in a area of A = 0.00196 m. Observe the specimen under both low and high power. Protect it from room light: Term. Specifically, in clinical medicine, histopathology refers to the study of a biopsy or surgical specimen by a pathologist after the specimen. specimen in tension or compression. c. The Amylase assay acts on hemoglobin. a. Hemolysis b. Leukopenia c. Decreased platelets d. Increased hematocrit, You run an ELISA for molecule X. a. Cell density. d. blood cultures. Type of specimen required for rapid strep test, Examples: Which of the following options is correct? Allow the sample to remain in the furnace for one hour. Often applied to metallic elements, specific heat can be used as a basis for comparing how different substances absorb and transfer energy. Cold agglutinin A physician orders a test you're unfamiliar with. What is the best way to chill a specimen? At the steady state, heat conducted through the bad conductor is equal to heat radiated from the Lees disc. Now put all the values in the formula. https://blog.btlabsystems.com/blog/when-to-use-a-heat-block With a unique design, compact size and small footprint, all BT Lab Systems heat blocks are lightweight and can be placed on a benchtop or flat surface in any laboratory setting. d. an immature blood cell. Glucose test specimens drawn in sodium fluoride tubes are stable for how when refrigerated? See picture. Jun 4, 2018. No change in the appearance of blood cells as they have not been digested. Find the speed of the m th-order maxima on the screen, where m can be very large. Suspected agents should include N. meningitidis, S. pneumoniae, and H. influenzae and other pathogens in some cases. Specimen processing and storage variables. Merelani Hills, Lelatema Mtns., Simanjiro Dist., Manyara Region, Tanzania. b. Void into toilet, void into container, void any remaining urine into toilet. Its a safe and convenient tool used to heat samples in flasks, tube, and vials, which is ideal for obtaining consistent results and precise temperature stability. Fill in the blanks with correct answers. This test can be used to evaluate stomach acid production. placing the specimen in a portable heating block, wrapping the specimen tube with a heel warmer. Once the slide is heat fixed, it can then be stained. What other labs should be ordered for her? Red top clot activator tube. Longer filament path. Small Cabinet, 6.6 x 2.2 x 1.8 cm. The compressive strength testing machine consist of two steel bearing blocks, one is in rigid position on which the masonry unit is placed and . Given that the molecular formula of sand is SiO_2 and that its specific heat is 0.84 J/g degree C, its molar heat capacity is: a. c. cholesterol Monitor the temperature of the control bottle closely and begin timing when the temperature reaches 562C. So, 1676 KJ = 1,000 1676 = 16,76,000 J. Serial urine specimens collected at specific times for C & S. Which urine spec is obtained by inserting a sterile needle directly into the urinary bladder and aspirating urine? Which of the following variables represents a preanalytic variable that might affect laboratory testing results? when the specimen is transported, processed and tested. 5. The container must be red in color Again, I wonder how the big guys do it! 3b, according to the diffraction peak, the heat-treated high chromium cast iron sample is mainly composed Heat generated causes marked alteration in both Let final temperature of mixture = T C. The analog of is current, and the analog of the temperature difference, , is voltage difference. 8 hrs. a. biohazard bag b. shaking the tube to mix the contents Heat. What specimen preferred for most urine tests? B antigens on the red blood cells b. Specimen Identification Confidence each patient is getting the right diagnostic report comes through ensuring chain of custody from sample, to slide, to diagnosis. Most likely reason a Culture + Sensitivity be ordered? Why must you transport tubes with stopper up? Heat the boiler using steam from the steam generator (refer to the fig.1) Figure 1 6. Change in heat of iron Qiron = 200 1000 450 (T 300) J. A patient needs a blood transfusion. Question 1 Angioplasty and a stent would most likely be used to treat - hemophilia. How do you preserve a urine cytology specimen? Serial urine specimens collected at specific times for C & S. Which urine spec is obtained by inserting a sterile needle directly into the urinary bladder and aspirating urine? What type of test was most likely ordered? Ball screen. The phlebotomist drew a blood specimen and placed it in a {eq}37 ^\circ C a. Cell density. Submit paraffin blocks or wet tissue (tissue fixed in formaldehyde, Bouin's solution or B-5 suitable). copyright 2003-2023 Homework.Study.com. The agglutination, lateral flow tests, and ELISA can be used to test for O157 and H7 antigens (agglutination, lateral flow) and Shiga toxin (ELISA). b. blood gases Correct filling and mixing of the specimen tubes, specimen leakage, tube breakage, excessive shaking. Which of the following tests might it have been? Fixing is a necessary step when performing a stain on a slide, explains Ohio State professor Stephen Abedon. Most commonly tests for glucose and ketones. Disadvantages include inability to cut thin sections, storage of blocks in alcohol and speed of technique (which can take several weeks or months). Which of the following is least likely to have caused the hemolysis? Heat fixing bacterial smears kills the bacterial cells so that they are fixed in place and ready for staining. unique number generated when a test request is entered into computer. b. The use of the rocker is to ensure that the samples are adequately mixed after thawing. Lets learn more. CLSI guidelines sets the maximum time limit for separating serum and plasma fro the cells at how long? Mix by gentle inversion 3-4 times. 30 Q Which of the following specimens requires chilled transport? Why? gestational age The faceshell thickness is 30 mm and the web thickness is 30 mm. Cytomegalovirus d. Green top plasma. SCIENCE Paper 1. Excessive agitation of a specimen is likely to cause which of the following conditions? Credit Solution Experts Incorporated offers quality business credit building services, which includes an easy step-by-step system designed for helping clients build their business credit effortlessly. Why are these tests done? To protect from light. George holds faculty appointments with Rutgers University and Michigan State University, and is a member of the International Society on Thrombosis and Hemostasis, American Association of Clinical Chemists, and American Society for Clinical Laboratory Science. A: Yes, blood specimen collection tubes are in shortage, including sodium citrate tubes. sleep. The capillary tubes that are used to draw blood for the measurement of hematocrit are treated with heparin. Fixation is a critical step in preparing slides and samples in microbiology, pathology and histology. Allow the patient to check the labels on the blood specimen. Cambridge Assessment International Education Official Website To fight heat creep, you must understand why this is happening. 22. which specimen was in the heat block why. When sections are made and processed, the ink will mark the actual margin on the slide. The laser beam passes through a pair of slits separated by a distance d, in a glass plate attached to the front of the laser. It is used with PCR tubes, PCR strips, Greiner tubes, microplates and cuvettes. Ft. 36071 Montezuma Valley Rd, Ranchita, CA 92066. To speed the process . Challenges are also present in interpreting the 1.1 (a)(i) On Fig. So, the conversion is like this. At Clarian we have always used a 37Cwater bath to thaw our frozen coagulation samples and then mix well for 3-5minutes on a mechanical rocker. With a unique design, compact size and small footprint, all BT Lab Systems heat blocks are lightweight and can be placed on a benchtop or flat surface in any laboratory setting. b. hematocrit. Lighting examination b. Fluorometric assays c. Colorimetric assays d. ELISA, The final reaction in blood clotting is: A. Fibrinogen to Fibrin B. Platelets to Prothrombin activator C. Prothrombin to Thrombin D. Plasmin to Fibrin E. None of the above, Which of the following hematologic changes may result from the use of dapsone? 4 Use Figure 5 to determine the time taken for the stearic acid to change from a liquid to a solid. MAE 20 Winter 2011 Assignment 5 6.7 For a bronze alloy, the stress at which plastic deformation begins is 275 MPa (40,000 psi), and the modulus of elasticity is 115 GPa (16.7 106 psi). Accumulation of which of the following substances, usually cleared by the liver, results in hepatic encephalopathy? The specific heat is the amount of heat necessary to change the temperature of 1.00 kg of mass by 1.00C. b. leakproof plastic bag What do you do? A microprocessor-based closed-loop servo system can be implemented to accurately control the speed of the crosshead. pH/blood gas (CBG & ABG) a. What protective equipment is required when processing specimens? This type of specimen is obtained by inserting a flexible swab through the nose. I have never put specimens on a rocker, however, and am curious if you have found this improves their quality. I am glad to know that folks are using a rocker but keep in I am glad to know that folks are using a rocker but keep in mind that this should be done AFTER the sample has been thawed in a water bath at 37C. Heat block is a block that can melt snow and ice just like a torch except it does not give off light. The calorimeter measures the heat energy transferred. Glucose in a sodium fluoride tube can be stored at room temp for how long? Specimens that require separation of the serum or plasma from the cells should be centrifuged within how long? Based on available information, the FDA determined several types of blood specimen collection tubes are. What is phlebotomy? Lactic acid June 22, 2022 . which specimen was in the heat block why; nz herald morning quiz today; Thng Su 10, 2022 . Which of the following confirms the presence of active (reinfection) tuberculosis? The blocks should be tested with in 3days after collected in lab. A antibodies in the plasma c. A antigens on the red blood cells d. Rh antigen on the red blood cells e. A and B antibodies in the plasma, Bloodstains are found at the site of a murder. What is a portion of a specimen used for testing called? What type of specimen preferred for pregnancy testing? 1:30pm= 1330 "thirteen thirty", 10% dilution of bleach (5.25% sodium hypochlorite) a. reporting a critical lab value on the wrong patient Included PT-1000 temperature sensor allows temperature control directly in the sample vessel. When does a phlebotomists' job end in regard to handling a blood specimen? Many of the heat transfer processes encountered in industry involve composite systems and even involve a combination of both conduction and convection.Heat is first transferred from the hot fluid to the wall by convection, through the The most common reasons that metals undergo heat treatment are to improve their strength, hardness, toughness, ductility, and corrosion resistance. c. Blocks more light from passing through a specimen. The block is then sliced, put in a water bath (to remove any wrinkles in the sections), and eventually on the slide. A suture should be placed in one edge of the specimen (substitute for forceps for tissue immobilization). Junho. Is collected 2 hours after a meal and tests for glucose. 2022 PEOPLE BEHIND WECRUIT CHROMBIT ASIA. How is a catheterized urine specimen collected? which specimen was in the heat block whygirondins bordeaux players. Included PT-1000 temperature sensor allows temperature control directly in the sample vessel. A urinalysis reveals macroalbuminuria. e. Stain color. How would you interpret the following data? Serum is: a. blood solids. (A) O (B) AB (C) B (D) A. Select the correct answer. Must complete clotting (usually 30-60 minutes) at room temperature before centrifugation. The specimen was . What type of specimen preferred routine analysis? Chills due to the donor blood being colder than normal body temperature Kidney shut down due to too much blood being infused at once RBCs lyse and hemoglobin levels increase in the blood stream Dehydration due to incomp.
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